RT-PCR confirmation of expression of selected flagellar-related genes only in 1N cells. All reactions were run with the same RT+ cDNA samples. The RT-PCR shown at the top used the elongation factor 1α (GS000217) as a positive (loading) control showing successful cDNA amplification occurred in all samples. RT- control reactions prepared from the same RNA were run for nine of the PCRs shown here and no contaminating genomic DNA (gDNA) was ever found (see examples with RT- reactions included in Figure S6 in Additional data file 1). For clarity, RT- control reactions run simultaneously have been cut out here. Positions of molecular weight markers on each side of the gel are shown. The sample identifiers are listed for each lane at the top of the gel. 11 h, harvested at 11 h (late morning); 21 h, harvested at 21 h (early evening, time of S-phase); 02 h, harvested at 02 h (after cell division); CL, cultures (1N only) exposed to continuous light.
von Dassow et al. Genome Biology 2009 10:R114 doi:10.1186/gb-2009-10-10-r114