Comparison of OG1RF and V583 in a mouse urinary tract infection model. (a) Mixed infection by wild-type E. faecalis strains OG1RF and V583 in the kidneys and urinary bladders of mice (n = 21; competition assay). Data are expressed as the log10(CFU)/gm for OG1RF or V583; the log10(CFU)/gm for both kidneys were combined and averaged from two independent experiments. Black solid diamonds and triangles represent E. faecalis strains OG1RF and V583, respectively, for kidney homogenates, and empty diamonds and triangles represent OG1RF and V583, respectively, for urinary bladder homogenates. Horizontal bars represent geometric means. Log10(CFU) were compared for statistical significance by the paired t-test. The minimum detection limit in these experiments was 101 and 102 CFU/gm of kidney and urinary bladder homogenates, respectively. (b) Mono-infection using E. faecalis strains OG1RF or V583 in the kidneys of mice (103 CFU per mice, n = 9). Data are expressed as log10(CFU)/gm for OG1RF recovered from kidney homogenates 48 h after infection; the log10(CFU)/gm for a kidney pair were combined and averaged. Black and white triangles represent OG1RF and V583, respectively. Horizontal bars represent geometric means. The CFU of V583 recovered from kidneys was significantly reduced compared to the CFU of OG1RF, as determined by the unpaired t-test.
Bourgogne et al. Genome Biology 2008 9:R110 doi:10.1186/gb-2008-9-7-r110