TSS predictions are consistent with independent experimental data. MAPP predictions for the same strand as the studied gene are plotted above the genome annotation. (a) PF11_0009; (b) PF13_0011; (c) PF14_0323. The MAPP profile ranges from 0 to 1 (maximum). Red rectangles represent genes and arched lines represent introns. The genome is represented by the black line upon which each gene is centered. Blue arrows above the genome line represent TSSs from the FULL-Malaria database, while black arrows below the genome line are those that have been identified in other studies. Numbers above these arrows are the number of multiple TSS that could not easily be distinguished at the scale with individual arrows. In all cases, only one DNA strand is shown and directionality can be inferred from the direction of TSS arrows. The scale is given between the genome and the MAPP profile and is zeroed at the translation start site of the gene. In (c), the combined regions represented by the parentheses contain 47 individual TSSs. Those TSSs between the start codon and -80 nucleotides predominantly give rise to unspliced transcripts, while those in the region further upstream (to -172 nucleotides) give rise to correctly spliced mRNA.
Brick et al. Genome Biology 2008 9:R178 doi:10.1186/gb-2008-9-12-r178