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This article is part of the supplement: Transposons in vertebrate functional genomics

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Germline mutagenesis mediated by Sleeping Beauty transposon system in mice

Junji Takeda12*, Vincent W Keng23 and Kyoji Horie1

Author Affiliations

1 Department of Social and Environmental Medicine, Graduate School of Medicine, Osaka University, Yamadaoka, Suita, Osaka 565-0871, Japan

2 Center for Advanced Science and Innovation, Osaka University, Yamadaoka, Suita, Osaka 565-0871, Japan

3 Department of Genetics, Cell Biology, and Development, The Cancer Center, University of Minnesota, Minneapolis, Minnesota 55455, USA

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Genome Biology 2007, 8(Suppl 1):S14  doi:10.1186/gb-2007-8-s1-s14

Published: 31 October 2007


Following the descovery of its transposition activity in mammalian culture systems, the Sleeping Beauty (SB) transposon has since been applied to achieve germline mutagenesis in mice. Initially, the transposition efficiency was found to be low in cultured systems, but its activity in germ cells was unexpectedly high. This difference in transposition efficiency was found to be largely dependent on chromosomal status of the host genomic DNA and transposon vector design. The SB transposon system has been found to be suitable for comprehensive mutagenesis in mice. Therefore, it is an effective tool as a forward genetics screen for tagged insertional mutagenesis in mice.