Additional data file 1.

EBs were generated using the conventional hanging drop protocol (see Materials and methods and Figure 5b) and the expression of the T-bra, flk1, smooth muscle α-actin, cardiac Troponin T (c-Troponin T), NF-H, NF-M and AFP was detected using RT-PCR (for the conditions, see Additional data file 14).

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Doss et al. Genome Biology 2007 8:R184   doi:10.1186/gb-2007-8-9-r184