Additional data file 2.

The MLST analysis of EHEC strains of the present study with other pathogenic E. coli strains in the EcMLST database was conducted by using concatenated DNA sequences of seven loci (aspC, clpX, fadD, icdA, lysP, mdh and uidA). The sequences of reference strains were obtained from EcMLST. Multiple sequence alignments were made by using the ClustalW program in the MEGA3 software. The NJ tree was generated by using the Tamura-Nei evolutionary model. Bootstrap values greater than 50% are indicated. The scale bar represents the number of substitutions per site. Accession numbers in EcMLST, strain names, serotypes, and classes in EcMLST of each strain are indicated. MLST20 is an undefined clonal group.

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Ogura et al. Genome Biology 2007 8:R138   doi:10.1186/gb-2007-8-7-r138