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qBase relative quantification framework and software for management and automated analysis of real-time quantitative PCR data

Jan Hellemans, Geert Mortier, Anne De Paepe, Frank Speleman and Jo Vandesompele*

Genome Biology 2007, 8:R19  doi:10.1186/gb-2007-8-2-r19

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Error in Exponential Amplification equation (formula 5)?

Jamie McCall   (2015-07-10 16:58)  University of Nebraska Medical Center

We are having difficulty calculating reasonable exponential amplifications using Formula 5 in the paper. Is it supposed to have a negative sign before the 1 (ie. Ejl = 10 ^ (-1/slope))?

Competing interests



error in formula 4

Jo Vandesompele   (2013-02-26 16:42)  Ghent University Hospital

It only came recently to our attention that the factor (h-1) in the denominator of equation 4 misses a square root symbol. As such it should be sqrt(h-1).

Competing interests

None declared


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