Methods for quantifying relative protein and mRNA levels give similar results. Average expression of kni mRNA at the beginning of stage 5 (7 embryos) is compared to kni protein expression at mid-stage 5 (17 embryos). The two graphs show the expression along the a/p axis (x-axis) at the ventral (top graph) and dorsal (bottom graph) midlines. The levels of fluorescence for mRNA labeling and protein labeling have remarkably similar shapes. Egg length (EL).
Luengo Hendriks et al. Genome Biology 2006 7:R123 doi:10.1186/gb-2006-7-12-r123