Hydrophobicity histrogams. (a) Mean hydrophobicity histogram for peptides in the S. cerevisiae PeptideAtlas (darker hashed bars) and an in silico tryptic digest of the SGD reference protein database allowing one missed cleavage (lighter hashed bars). (b) The reference peptides' hydrophobicities divided by the observed peptides' hydrophobicities. The lowest hydrophobicity peptides are generally washed off the column in the reverse phase stage of the HPLC process and hence not measured.
King et al. Genome Biology 2006 7:R106 doi:10.1186/gb-2006-7-11-r106