Genomic analysis of heat-shock factor targets in Drosophila
- Equal contributors
1 Department of Anatomy, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK
2 Department of Genetics, University of Cambridge, Downing Street, Cambridge, CB2 3EH, UK
Genome Biology 2005, 6:R63 doi:10.1186/gb-2005-6-7-r63Published: 10 June 2005
We have used a chromatin immunoprecipitation-microarray (ChIP-array) approach to investigate the in vivo targets of heat-shock factor (Hsf) in Drosophila embryos. We show that this method identifies Hsf target sites with high fidelity and resolution. Using cDNA arrays in a genomic search for Hsf targets, we identified 141 genes with highly significant ChIP enrichment. This study firmly establishes the potential of ChIP-array for whole-genome transcription factor target mapping in vivo using intact whole organisms.