Predicted stem-loop structure of a novel mammalian microRNA, rno-miR-421. The stem-loop structure was predicted from sequences adjacent to rno-miR-421 in the rat genome. The cloned (mature) sequence is shown in red. The predicted secondary structure and the free energy calculation (ΔG, kcal/mole) were generated by the mfold software .
Miska et al. Genome Biology 2004 5:R68 doi:10.1186/gb-2004-5-9-r68