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Microarray-based genomic surveying of gene polymorphisms in Chlamydia trachomatis

Brian W Brunelle1, Tracy L Nicholson1 and Richard S Stephens12*

Author Affiliations

1 Program in Infectious Diseases, University of California, Berkeley, CA 94720-7360, USA

2 Francis I. Proctor Foundation, University of California, San Francisco, CA 94143-0412, USA

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Genome Biology 2004, 5:R42  doi:10.1186/gb-2004-5-6-r42

Published: 18 May 2004


By comparing two fully sequenced genomes of Chlamydia trachomatis using competitive hybridization on DNA microarrays, a logarithmic correlation was demonstrated between the signal ratio of the arrays and the 75-99% range of nucleotide identities of the genes. Variable genes within 14 uncharacterized strains of C. trachomatis were identified by array analysis and verified by DNA sequencing. These genes may be crucial for understanding chlamydial virulence and pathogenesis.