Microarray-based genomic surveying of gene polymorphisms in Chlamydia trachomatis
1 Program in Infectious Diseases, University of California, Berkeley, CA 94720-7360, USA
2 Francis I. Proctor Foundation, University of California, San Francisco, CA 94143-0412, USA
Genome Biology 2004, 5:R42 doi:10.1186/gb-2004-5-6-r42Published: 18 May 2004
By comparing two fully sequenced genomes of Chlamydia trachomatis using competitive hybridization on DNA microarrays, a logarithmic correlation was demonstrated between the signal ratio of the arrays and the 75-99% range of nucleotide identities of the genes. Variable genes within 14 uncharacterized strains of C. trachomatis were identified by array analysis and verified by DNA sequencing. These genes may be crucial for understanding chlamydial virulence and pathogenesis.