Expression of MYC and direct Myc target genes in treated (+ tet) and untreated (- tet) P493 cells. (a) Left panel reflects Myc protein expression by western blot analysis. Alpha-tubulin is shown as a loading control. (b) Real-time RT-PCR using SYBR Green to detect mRNA levels of indicated genes in both treated (white bars) and untreated (black bars) P493 cells. 18S RNA was used as an internal standard. MYC and NPM1 are shown as positive controls.
Zeller et al. Genome Biology 2003 4:R69 doi:10.1186/gb-2003-4-10-r69