Experimental design and diagram of caste differentiation and development in Reticulitermes termites. Castes enclosed in blue boxes (soldier, worker) were compared initially by using cDNA macroarrays prepared from all stages shown except adult reproductives. Next, quantitative PCR was used to compare expression levels of specific gene transcripts between castes enclosed in blue boxes (soldier, worker) versus intermediary developmental stages enclosed in red boxes (nymph, presoldier).
Scharf et al. Genome Biology 2003 4:R62 doi:10.1186/gb-2003-4-10-r62