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Integrative genomics finds disease genes

Jonathan B Weitzman

Genome Biology 2003, 4:spotlight-20030114-01  doi:10.1186/gb-spotlight-20030114-01

The electronic version of this article is the complete one and can be found online at:

Published:14 January 2003

© 2003 BioMed Central Ltd

Research news

In the Early Edition of the Proceedings of the National Academy of Sciences Mootha et al. describe an integrative genomic approach to discover a gene associated with the human cytochrome c oxidase (COX) deficiency, Leigh syndrome, French Canadian type (LSFC). LSFC is one of five autosomal recessive COX deficiencies; the other four are due to defects in genes encoding COX assembly factors. Mootha et al. combined experimental data from genomic, transcriptome and proteomic studies to identify the gene causing LSFC. They systematically analysed potential genes in the LSFC candidate region that had been narrowed down to a 2 megabase genomic region on chromosome 2p16-21. Neighborhood analysis of large-scale microarray data identified genes that are co-regulated with mitochondrial genes; this was combined with organelle-specific mass spectrometry proteomic data. Integrating these DNA, mRNA and protein-based data led to the identification of a promising candidate gene, LRPPRC (leucine-rich pentatricopeptide repeat-containing protein). Mootha et al. found mutations in LRPPRC in patients with LSFC, validating their integrative genomic approach to disease-gene discovery.


  1. [] webcite

    Proceedings of the National Academy of Sciences

  2. [] webcite

    Identification of a gene causing human cytochrome c oxidase deficiency by integrative genomics

  3. [] webcite

    Leigh syndrome, French Canadian type

  4. Cytochrome c oxidase deficiency.

    PubMed Abstract | Publisher Full Text OpenURL