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Proteome purification

Jonathan B Weitzman

Genome Biology 2002, 3:spotlight-20020308-01  doi:10.1186/gb-spotlight-20020308-01

The electronic version of this article is the complete one and can be found online at:

Published:8 March 2002

© 2002 BioMed Central Ltd

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In the March 5 Proceedings of the National Academy of Sciences, Braun et al. describe a methodology to perform high-throughput purification of human proteins using several affinity-tags (Proc Natl Acad Sci USA 2002, 99:2654-2659). They exploited the Gateway recombinational cloning system that employs a versatile 'master' vector for easy transfer into different expression vectors. They chose a test set of 32 human genes and expressed them in four different vectors with different affinity tagsL: the His6tag, calmodulin-binding peptide, glutathione-S-transferase or maltose-binding protein. Using different denaturing and nondenaturing purification conditions they were able to isolate around 80% of expressed proteins. Braun et al. demonstrated that the system can be easily scaled-up, and then applied the same approach to a set of 336 human cDNAs with a success rate of around 60%. This approach allows for optimization of protein purification on a proteome-scale.


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