Figure 2.

'Full-cycle' rRNA approach to characterizing microorganisms in their natural settings without the need for cultivation. Access to whole genomes of uncultivated organisms is also possible using the same basic approach but with large-insert cloning vectors, such as BACs, which remove the need for PCR.

Hugenholtz Genome Biology 2002 3:reviews0003.1-reviews0003.8   doi:10.1186/gb-2002-3-2-reviews0003