Figure 6.

Annotation of the light (lt) region. The 594-kb WGS3 scaffold 211000022280798 and twelve curated gene models are shown. The WGS3 sequence is shown as a bar with sequence gaps (black), transposable elements and simple repeats that were masked and removed during the annotation process (red), and presumed single-copy sequences that remained after masking (gray) indicated. Gene models are shown as blue bars with exons (thick) and introns (thin) indicated. Those above the line are transcribed on the forward strand, and those below the line are transcribed on the reverse strand. The average density of curated genes is one per 50 kb, about six- to sevenfold lower than the density in the euchromatin [12,48]. Only the lt and cta genes are identified by genetic analysis. Seven gene models were described in the Release 1 annotation [12]. This annotation provides a more accurate view of the structures of nearly all of the gene models and determines their relative locations. cDNA sequence alignment allowed us to merge two Release 2 gene models, Chitinase 1 and 3, into a single gene Cht3 with multiple chitin-binding and catalytic domains. Two of the three new curated genes (CG40006, CG40016) are represented by multiple ESTs and cDNAs. CG40005 is based solely on BLAST evidence; its similarity to the adjacent cta gene suggests a possible sequence assembly artifact. On the basis of the masking results, known transposable element sequences account for 302 kb (51%) of the sequence scaffold.

Hoskins et al. Genome Biology 2002 3:research0085.1-0085.16   doi:10.1186/gb-2002-3-12-research0085