Relationship between the Cy5/Cy3 fluorescence ratios measured using antigen microarrays and the concentration ratio of the cognate antibodies. The median of the log10 transformed Cy5/Cy3 fluorescence ratios of the indicated antigens from Figure 2 were plotted as a function of concentration ratio, as in Figure 3. Median values from the replicate spots are presented along with the concentration ratio of the cognate antibody, represented by the dashed line. The error bars represent the standard deviation between the replicate spots.
Haab et al. Genome Biology 2001 2:research0004.1-research0004.13 doi:10.1186/gb-2001-2-2-research0004