Email updates

Keep up to date with the latest news and content from Genome Biology and BioMed Central.

Open Badges Research news

Gastric interactions

William Wells

Genome Biology 2001, 2:spotlight-20010117-01  doi:10.1186/gb-spotlight-20010117-01

The electronic version of this article is the complete one and can be found online at:

Published:17 January 2001

© 2001 BioMed Central Ltd

Research news

Large numbers of protein-protein interactions have been mapped for yeast and worms, and now in the January 11 Nature, Rain et al. present the first large set of interactions for a prokaryote (Nature 2001, 409:211-215). The two-hybrid screen of 261 proteins from the gastric pathogen Heliobacter pylori against a library of genome-encoded polypeptides revealed 1,200 putative interactions. Screening against a library allows the identification of interacting domains, and reduces the rate of false negatives encountered in classical pair-wise screens. A strong selection protocol reduces the number of false positives. Rain et al. also use a probability score to compute the likelihood that a given two-hybrid result is a consequence of background noise, and use some of the identified interactions to assign various proteins to particular biological pathways.


  1. A comprehensive analysis of protein-protein interactions in Saccharomyces cerevisiae.

    PubMed Abstract | Publisher Full Text OpenURL

  2. Protein interaction mapping in C. elegans using proteins involved in vulval development.

    PubMed Abstract | Publisher Full Text OpenURL

  3. [] webcite