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Metabolite profiling

William Wells

Genome Biology 2000, 1:spotlight-20001108-03  doi:10.1186/gb-spotlight-20001108-03

The electronic version of this article is the complete one and can be found online at:

Published:8 November 2000

© 2000 BioMed Central Ltd

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In the November Nature Biotechnology Fiehn et al. offer an alternative to the profiling of messenger RNA and protein levels. They use gas chromatography coupled to mass spectrometry (GC/MS) to assay the relative levels of 326 small compounds from a plant leaf extract (Nat Biotech 2000, 18:1157-1161). A simple methanol extraction is followed by derivitization to increase metabolite stability and volatility. Approximately half of the chromatographed compounds can be identified based on retention times and mass spectra; these results can be viewed on the accompanying website. Biological variability (of approximately 40%) is in clear excess of variability inherent to the method (about 8%). Principal component analysis allows metabolite profiles from plants of a particular genetic background or with a particular mutation to be clustered. Mutation of a single gene causes many changes, most of them unexplained. Metabolite profiles may be useful to address public concerns about the safety of genetically modified food.


  1. [] webcite

    Nature Biotechnology

  2. Metabolic profiling: a Rosetta Stone for genomics?

    PubMed Abstract | Publisher Full Text OpenURL

  3. [] webcite

    Metabolite Mass Spectra Library