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Microarrays for secreted proteins

William Wells

Genome Biology 2000, 1:spotlight-20000428-02  doi:10.1186/gb-spotlight-20000428-02

The electronic version of this article is the complete one and can be found online at:

Published:28 April 2000

© 2000 BioMed Central Ltd

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Secreted and membrane-associated proteins are important drug targets, but algorithms for recognizing the corresponding genes are imperfect, especially when the entire coding sequence is not available. Diehn et al. report in the May Nature Genetics that these proteins can be catalogued in two easy steps (NatureGenet. 2000, 25:58-62). Diehn et al. isolate membrane-bound mRNAs (attached to polysomes) and cytosolic mRNAs, followed by hybridization of the corresponding cDNAs to DNA microarrays. The majority of the known mRNAs that are enriched in the membrane fraction encode for secreted or membrane-bound proteins. Based on this correspondence, Diehn et al. claim to have identified over 275 human genes and 285 yeast genes that are likely to encode previously unrecognized secreted or membrane proteins.


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