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Analysis of protein sequences

Mar Albà

Genome Biology 2000, 1:reports225  doi:10.1186/gb-2000-1-1-reports225

The electronic version of this article is the complete one and can be found online at:

Received:23 November 1999
Published:17 March 2000

© 2000 BioMed Central Ltd

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Several programs to predict sequence and structural features of a protein can be run simultaneously on the PredictProtein server. The programs have been contributed by various authors. MaxHom is a program that searches databases for homologous proteins using BLAST and performs a multiple alignment of the most closely related proteins. Homology to known protein domains is investigated by running searches against ProSite and ProDom. PHDsec and PHDacc are used to predict secondary structure and solvent accessibility, respectively. Other structural characteristics that can be investigated are globularity (Globe), transmembrane helices (PHDhtm), coiled-coil regions (Coils) and fold (Topits).


The simple structure of the site, with three main submission forms (default, advanced, expert) makes navigation easy. In fact, the server would benefit from more links to files with information on the algorithms and basic features of the different programs. Instead, such information partially appears in the output files, making them unnecessarily long.

Reporter's comments


It was not clear when the site was last updated, but the SWISS-PROT version used to do BLAST searches was up to date (release 38.0, July 1999).

Best feature

Different characteristics of a protein can be investigated in a matter of minutes. A very useful feature is the construction of multiple alignment from BLAST hits by MaxHom.

Worst feature

The output options are not well organized so it is not always clear which programs are running.

Wish list

It would be useful to be able to get the results directly from the web browser, instead of their being sent by e-mail. Also, the service would improve by adding other databases, besides SWISS-PROT, to perform BLAST searches.

Related websites

More on protein prediction can be found at Expasy and the Protein sequence analysis protein structure prediction server.

Table of links

Assumptions made about all sites unless otherwise specified:
The site is free, in English and no registration is required. It is relatively quick to download, can be navigated by an 'intermediate' user, and no problems with connection were found. The site does not stipulate that any particular browser be used and no special software/plug-ins are required to view the site. There are relatively few gratuitous images and each page has its own URL, allowing it to be bookmarked.