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Ribozymes as tools for the analysis of gene function

Ines Chyla

Genome Biology 2000, 1:reports208  doi:10.1186/gb-2000-1-1-reports208

The electronic version of this article is the complete one and can be found online at:

Received:20 October 1999
Published:17 March 2000

© 2000 BioMed Central Ltd


Valuable information on the use of ribozymes as tools for the analysis of gene function can be found here. How ribozymes function to perturb gene expression is discussed in detail. A review article focuses on the use of ribozymes as specific inhibitors of viral and cellular gene expression, both in tissue culture cells and at the level of the whole organism. The review covers the role of cellular factors, such as RNA-binding proteins, as possible facilitators of ribozyme action within cells. A computational approach to identifying possible target sites for ribozyme action is also described.


It is relatively easy to navigate through the site as it consists of only three pages. There are, however, no 'back to...' links so the back button of the browser has to be used instead. The external links for the software program described do not work, and neither do some of the links to cited literature. None of the small images within the text displayed, but the links to the larger versions of the images all worked.

Reporter's comments


The site was last updated 30 April 1997.

Best feature

This is the best comprehensive information on ribozymes available on-line, and is supported by explanatory graphics.

Worst feature

Apart from the lack of updates, there is no real site structure and many links are broken. Some of the graphics are too large to be printed without becoming truncated.

Wish list

The site needs a thorough update to repair the broken links, especially the external ones. It would also benefit from the addition of links to other important papers on the subject, and a general update to include recent developments.

Table of links

Assumptions made about all sites unless otherwise specified:
The site is free, in English and no registration is required. It is relatively quick to download, can be navigated by an 'intermediate' user, and no problems with connection were found. The site does not stipulate that any particular browser be used and no special software/plug-ins are required to view the site. There are relatively few gratuitous images and each page has its own URL, allowing it to be bookmarked.